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primary antibodies against cd36 sc-7309  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibodies against cd36 sc-7309
    Primary Antibodies Against Cd36 Sc 7309, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+for+cd36/anti+cd36/pmc10743360__pnas__2304213120__sapp-162-7-11
    Average 90 stars, based on 1 article reviews
    primary antibodies against cd36 sc-7309 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression
    Article Snippet: .. Cells were incubated with primary antibodies for CD36 (Santa Cruz # sc-7309) and MMP28 (Abcam #ab175937) in 1% BSA for 2 h at room temperature. .. Cells were then washed 3X with PBS and incubated with fluorescent secondary antibodies (Thermo Fisher#) in 1% BSA for 1 h at room temperature.

    Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets
    Article Snippet: The gel was transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 ◦C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech). .. Subsequently, the membranes were washed with TBST (TBS with 0.1 % Tween-20) three times and incubated at room temperature for 2 h with a second antibody (Santa Cruz Biotechnology).

    Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets
    Article Snippet: The gel was transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech). .. Subsequently, the membranes were washed with TBST (TBS with 0.1 % Tween-20) three times and incubated at room temperature for 2 h with a second antibody (Santa Cruz Biotechnology).

    Blocking Assay:

    Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets
    Article Snippet: The gel was transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 ◦C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech). .. Subsequently, the membranes were washed with TBST (TBS with 0.1 % Tween-20) three times and incubated at room temperature for 2 h with a second antibody (Santa Cruz Biotechnology).

    Article Title: Triglyceride-lowering effect of rice protein due to the regulation of fatty acid uptake and transport of triglyceride in rats fed normal/oil-enriched diets
    Article Snippet: The gel was transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. After blocking with 5 % fat-free milk in TBS at room temperature for 1 h, the membranes were incubated overnight at 4 °C with primary antibodies for CD36 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MTP (Santa Cruz Biotechnology), FATP-2 (Proteintech, Wuhan, China), FABP-1 (Proteintech), lipoprotein lipase (LPL, Santa Cruz Biotechnology), Niemann-Pick C1-like 1 (NPC1L1, Santa Cruz Biotechnology), β-actin (Cell Signaling, Danvers, MA, USA) and GAPDH (Proteintech). .. Subsequently, the membranes were washed with TBST (TBS with 0.1 % Tween-20) three times and incubated at room temperature for 2 h with a second antibody (Santa Cruz Biotechnology).

    other:

    Article Title: TREM2 promotes Aβ phagocytosis by upregulating C/EBPα-dependent CD36 expression in microglia
    Article Snippet: The cells were incubated with primary antibodies for NeuN (1:1000; Millipore, Marlborough, MA, cat: MAB377), TREM2 (1:500; Abcam, cat: ab86491) and CD36 (1:500; Santa Cruz Biotechnology, cat: sc-7309) at room temperature for 24 h. After washing three times with PBS containing 0.1% Triton X-100 (Sigma), the cells were incubated with appropriate Alexa 488- or 560-conjugated secondary antibodies (1:2000; Thermo Fisher Scientific, Waltham, CA) for 1 h and counterstained with DAPI, followed by mounting with VectaShield mounting medium (Vector Laboratories, Burlingame, CA).



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    Detection of protein expression and functional verification of <t>CD36</t> and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
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    Detection of protein expression and functional verification of <t>CD36</t> and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
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    Detection of protein expression and functional verification of <t>CD36</t> and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
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    Detection of protein expression and functional verification of <t>CD36</t> and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
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    Image Search Results


    Detection of protein expression and functional verification of CD36 and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling

    doi: 10.3389/fimmu.2026.1636331

    Figure Lengend Snippet: Detection of protein expression and functional verification of CD36 and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.

    Article Snippet: Subsequently, primary antibodies against CD36 (18836-1-AP, Sanying, China) or KIT (AF6153, Affinity, USA) were added, and the sections were incubated overnight at 4°C followed by incubation with HRP-conjugated secondary antibody (K5007, Dako, Denmark) at 37 °C for 30 minutes.

    Techniques: Expressing, Functional Assay, Immunohistochemical staining, Staining, Migration

    Correlation analysis of CD36, KIT with TME and PA. (A) Detection of IFN-γ secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (B) Detection of TNF-α secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001.**** denotes P < 0.0001. (C) Detection of IL-10 secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors.** denotes P < 0.01. *** denotes P < 0.001. (D) Immunofluorescence staining results of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (E) Immunofluorescence staining results of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (F) Differential analysis of immunofluorescence optical density of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (G) Differential analysis of the ratio of nuclear to total immunofluorescence optical density of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors.**** denotes P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling

    doi: 10.3389/fimmu.2026.1636331

    Figure Lengend Snippet: Correlation analysis of CD36, KIT with TME and PA. (A) Detection of IFN-γ secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (B) Detection of TNF-α secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001.**** denotes P < 0.0001. (C) Detection of IL-10 secretion level in MKN45 cells after treatment with CD36 and KIT inhibitors.** denotes P < 0.01. *** denotes P < 0.001. (D) Immunofluorescence staining results of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (E) Immunofluorescence staining results of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors. (F) Differential analysis of immunofluorescence optical density of PARP-1 in MKN45 cells after treatment with CD36 and KIT inhibitors. *** denotes P < 0.001. (G) Differential analysis of the ratio of nuclear to total immunofluorescence optical density of AIFM1 in MKN45 cells after treatment with CD36 and KIT inhibitors.**** denotes P < 0.0001.

    Article Snippet: Subsequently, primary antibodies against CD36 (18836-1-AP, Sanying, China) or KIT (AF6153, Affinity, USA) were added, and the sections were incubated overnight at 4°C followed by incubation with HRP-conjugated secondary antibody (K5007, Dako, Denmark) at 37 °C for 30 minutes.

    Techniques: Immunofluorescence, Staining